1985•International Journal of Systematic BacteriologyRequires access

Monoclonal Antibody for an Outer Membrane Lipoprotein of the Pseudomonas fluorescens Group of the Family Pseudomonadaceae

Lucy M. Mutharia, Robert E. W. Hancock

Abstract

Monoclonal antibody MAI-6 directed against Pseudomonas aeruginosa strain PAO1 outer membrane lipoprotein H2 also interacted with a protein having an identical molecular weight from 50 of 52 strains of P. aeruginosa tested. This antibody interacted with the P. fluorescens group of the family Pseudomonadaceae and with Azotobacter vinelandii but not with several other species of gram-negative bacteria. A colony blotting assay performed with this antibody allowed rapid detection of the P. fluorescens-A. vinelandii group.

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Key concepts: Pseudomonas fluorescens, Pseudomonadaceae, Azotobacter vinelandii, Microbiology, Monoclonal antibody, Pseudomonadales, Pseudomonas aeruginosa, Bacteria