2002•European Journal of Mass SpectrometryRequires access

Detection and Characterization of Natural Polyamines by High-Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization (Electrospray Ionization) Mass Spectrometry

Sergiy Chesnov, Laurent Bigler, Manfred Hesse

Abstract

The application of high-performance liquid chromatography on-line coupled with atmospheric-pressure chemical-ionization mass spectrometry (HPLC-APCI-MS), electrospray-ionization mass spectrometry (ESI-MS), as well as with tandem mass spectrometry (MS/MS), for the analysis of natural products is discussed. The separation, detection and structure elucidation of polyamine-containing compounds contained in plant material and spider venoms was performed by means of these techniques. The use of the methods for biochemical investigations of diverse natural materials is proposed.

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Available abstract

Key concepts: Atmospheric-pressure chemical ionization, Direct electron ionization liquid chromatography–mass spectrometry interface, Mass spectrometry, Chemistry, Electrospray ionization, Chromatography, Extractive electrospray ionization, Sample preparation in mass spectrometry