1957•The Journal of ProtozoologyRequires access
Purification, Axenic Cultivation, and Description of a Soil Amoeba, Acanthamoeba sp.
R. Neff
Open publisher page 163 citations
Abstract
SUMMARY. A small soil amoeba was purified by an agar‐surface migration method. The amoeba was grown axenicly in a liquid medium composed of 1% proteose peptone, 1% glucose, and inorganic salts. The amoeba was identified as a species of Acanthamoeba. A hypothesis on the mechanism of agar‐surface purification of amoebæ is proposed. The nutritional requirements of this isolate are discussed; it is concluded that this organism metabolizes glucose.