2015•Journal of Clinical MicrobiologyOpen access

Multiplex Nucleic Acid Suspension Bead Arrays for Detection and Subtyping of Filoviruses

Cecilia Bergqvist, Petra Holmström, Gunnel Lindegren, Nina Lagerqvist, Mikael Leijon, Kerstin I. Falk

Abstract

Here we describe multiplex suspension bead array systems that allow fast and reliable detection of reverse transcriptase (RT) PCR amplified filovirus genomes and also enable subtyping of Ebola virus species and Marburg virus strains. These systems have an analytical sensitivity equivalent to that of RT-PCR.

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Key concepts: Subtyping, Multiplex, Virology, Biology, Multiplex polymerase chain reaction, Ebola virus, Nucleic acid, Genome