1030 THE EFFECT AND TOLERABILITY OF ALLOGENIC ADIPOSE-DERIVED STEM CELL ON ERECTILE DYSFUNCTION INDUCED BY CAVERNOUS NERVE INJURY

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (II)1 Apr 20131030 THE EFFECT AND TOLERABILITY OF ALLOGENIC ADIPOSE-DERIVED STEM CELL ON ERECTILE DYSFUNCTION INDUCED BY CAVERNOUS NERVE INJURY Su Jin Kim, Woong Jin Bae, Yong Sun Choi, Hyuk Jin Cho, Sung Hoo Hong, Ji Youl Lee, Tae-Kon Hwang, and Sae Woong Kim Su Jin KimSu Jin Kim Seoul, Korea, Republic of More articles by this author , Woong Jin BaeWoong Jin Bae Seoul, Korea, Republic of More articles by this author , Yong Sun ChoiYong Sun Choi Seoul, Korea, Republic of More articles by this author , Hyuk Jin ChoHyuk Jin Cho Seoul, Korea, Republic of More articles by this author , Sung Hoo HongSung Hoo Hong Seoul, Korea, Republic of More articles by this author , Ji Youl LeeJi Youl Lee Seoul, Korea, Republic of More articles by this author , Tae-Kon HwangTae-Kon Hwang Seoul, Korea, Republic of More articles by this author , and Sae Woong KimSae Woong Kim Seoul, Korea, Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.615AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The efficacy of autologus and syngenic adipose derived stem cell (ADSC) on the treatment of erectile dysfunction (ED) is well established, however, there was a little report using allogenic ADSC. Allogenic ADSC seems to have more benefits because stable supply of cells of uniform quality or use in an emergency are possible compared with autologus ADSC. Therefore, we studied about the effect and safety of allogenic ADSC on ED induced by bilateral cavernous nerve crushing injury. METHODS Male Sprague Dawley Rats (SD rats) were used: control group (Group I), bilateral cavernous nerve crushing group (Group II), administration with ADSC of SD rat into the major pelvic ganglion (MPG) after BCNC (syngenic; Group III), and administration with ADSC of wistar rats into the MPG adter BCNC (allogenic; Group IV). After 4-week, functional examination was done. MPG and corpus cavernosum were collected. Immunofluorescent staining of ADSCs was performed to compare the implantation degree. The degree of T-cell infiltration was compared to evaluate immune response. Western blot analysis of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) were done in corpus cavernosum. RESULTS Intracavernosal pressure (ICP) /mean arterial pressure (MAP) ratios of group III and IV were similar and significantly increased compared with group I. In MPG, the number of implanted ADSC of wistar rat was lower than ADSCs of SD rat, however, there was no significance. Moreover, the degree of T-cell infiltration was not significantly different. Significant increase expression of eNOS and nNOS were observed in group III and IV compared with group II. CONCLUSIONS The erectile function in the rats with allogenic ADSC administration was well preserved. And allogenic ADSC does not lead to severe immune response after administration. Therefore, it is possible to apply allogenic ADSC for the treatment of ED using stem cell. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e422 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Su Jin Kim Seoul, Korea, Republic of More articles by this author Woong Jin Bae Seoul, Korea, Republic of More articles by this author Yong Sun Choi Seoul, Korea, Republic of More articles by this author Hyuk Jin Cho Seoul, Korea, Republic of More articles by this author Sung Hoo Hong Seoul, Korea, Republic of More articles by this author Ji Youl Lee Seoul, Korea, Republic of More articles by this author Tae-Kon Hwang Seoul, Korea, Republic of More articles by this author Sae Woong Kim Seoul, Korea, Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...