2015Petroleum Science and TechnologyRequires access

Bioremediation of Crude Oil Contaminated Soil

Guangxu Yan, Bin Cai, Chunlin Chen, Yong Yue, Q. Wang, Haixin Deng, S. Liu, Shaobin Guo

Open publisher page 18 citations

Abstract

A laboratory study was undertaken to bioremediation crude oil contaminated soil by isolated strains. Pseudomonas strain A as the most superior indigenous biodegrader was selected. At the optimal growth condition of pH 7 and with 5 g/L NaCl, cosubstrate α-lactose, and inorganic salt FeSO4, 82.3% of crude oil degradation efficiency was obtained. Furthermore, characteristics of chemical composition during the biotreatment indicated that saturation hydrocarbons and some aromatics were effectively removed by strain A. N-alkane in crude oil was preferentially degraded, especially those in the range of nC16–nC24. Biodegradation of aromatics depended on benzene ring numbers and complexity of structures.

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What this paper is about

A laboratory study was undertaken to bioremediation crude oil contaminated soil by isolated strains. Pseudomonas strain A as the most superior indigenous biodegrader was selected. At the optimal growth condition of pH 7 and with 5 g/L NaCl, cosubstrate α-lactose, and inorganic salt FeSO4, 82.3% of crude oil degradation efficiency was obtained. Furthermore, characteristics of chemical composition during the biotreatment indicated that saturation hydrocarbons and some aromatics were effectively removed by strain A. N-alkane in crude oil was preferentially degraded, especially those in the range of nC16–nC24. Biodegradation of aromatics depended on benzene ring numbers and complexity of structures.

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Available abstract

A laboratory study was undertaken to bioremediation crude oil contaminated soil by isolated strains. Pseudomonas strain A as the most superior indigenous biodegrader was selected. At the optimal growth condition of pH 7 and with 5 g/L NaCl, cosubstrate α-lactose, and inorganic salt FeSO4, 82.3% of crude oil degradation efficiency was obtained. Furthermore, characteristics of chemical composition during the biotreatment indicated that saturation hydrocarbons and some aromatics were effectively removed by strain A. N-alkane in crude oil was preferentially degraded, especially those in the range of nC16–nC24. Biodegradation of aromatics depended on benzene ring numbers and complexity of structures.

Key concepts: Bioremediation, Biodegradation, Chemistry, Crude oil, Soil contamination, Environmental remediation, Petroleum, Contamination

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