Cloning of a gene from Streptomyces species complementing argG mutations.
Hiroshi Ishihara, Michiko Nakano, Hiroshi Ogawara
Abstract
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Hiroshi Ishihara, Michiko Nakano, Hiroshi Ogawara
Abstract
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A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 complemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.
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A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 complemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.
Key concepts: Streptomyces coelicolor, Biology, Plasmid, Cloning (programming), Genetics, Streptomycetaceae, Mutant, Streptomyces