2005•Journal of Clinical MicrobiologyOpen access

Comparison of Conventional Susceptibility Testing, Penicillin-Binding Protein 2a Latex Agglutination Testing, and mecA Real-Time PCR for Detection of Oxacillin Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococcus

Melissa B. Miller, Heather Meyer, Elaine Rogers, Peter H. Gilligan

Abstract

Penicillin-binding protein (PBP) 2a latex agglutination was compared with conventional susceptibility testing and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus. Inoculum volume and induction with oxacillin were PBP 2a testing variables. For coagulase-negative Staphylococcus, an increased inoculum volume of 10 microl greatly reduced the number of isolates requiring induction.

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Key concepts: Latex fixation test, Staphylococcus aureus, Microbiology, Coagulase, Penicillin, Staphylococcus, Penicillin binding proteins, Micrococcaceae