1983•European Journal of BiochemistryOpen access

Aberrant Migration of Lipopolysaccharide in Sodium Dodecyl Sulfate/Poyacrylamide Gel Electrophoresis

Penny J. Hitchcock

Abstract

Purified lipopolysaccharides of salmonellae strains were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Pre-electrophoresis of polyacrylamide gels had no apparent effect on one-dimensional silver-stained lipopolysaccharide profiles. However, without pre-electrophoresis, two-dimensional and three-dimensional patterns contained numerous bands with varied migration patterns compared to those in the one-dimension gels. The lipopolysaccharide was altered within the polyacrylamide gel during electrophoresis. Pre-electrophoresis of gels eliminated aberrant migration patterns.

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Key concepts: Polyacrylamide gel electrophoresis, Gel electrophoresis, Sodium dodecyl sulfate, Gel electrophoresis of proteins, Electrophoresis, Two-dimensional gel electrophoresis, Chemistry, Lipopolysaccharide