Development of a Selective Extraction Method for Pravastatin Quantification in Tablets using HPLC with Ultraviolet Detection

A high performance liquid chromatography method for estimation of pravastatin in tablets has been developed. The mobile phase consisted of acetonitrile and phosphates buffer in a volume percentage ratio of 7:3 v/v, pH 2.0 and was delivered at the rate of 1 mL/min and detected at 238 nm, retention times were approximately 7.3 min: this peak was analyzed with mass spectroscopy. The method was fully validated and validation parameters were: linearity range 10–200 ng/mL, correlation coefficient 0.999, mean recovery >99%, limit of quantification 5 ng/mL, and limit of detection 5 ng/mL; this method can be used for a quality control assay.