1997•Journal of Liquid Chromatography & Related TechnologiesRequires access

High Performance Liquid Chromatographic Determination of Taxol and Related Taxanes from Taxus Callus Cultures

Yan Wu, W. Zhu

Abstract

This study presents a protocol for analyzing taxol and five related taxanes from tissue culture samples of Taxus spp. by high performance liquid chromatography (HPLC) using a reverse phase C18 column. Sep-Pak C18 cartridges were used for semipurification of the crude extracts of the samples prior to analyses. Taxol, cephalomannine, 10-deacetyltaxol, 10-deacetylcephalo-mannine, baccatin III and 10-deacetylbaccatin III were well separated by the mobile phase of methanol : acetonitrile : water (25:35:45) at a flow rate of 1.0mL/min and detected at 227nm.

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Key concepts: Chemistry, Taxus, Chromatography, High-performance liquid chromatography, Methanol, Callus, Botany, Organic chemistry