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Degradation of pectic substances in green flax fibre by Erwinia carotovora

Odile Morvan, Alain Jauneau, Claudine Morvan, Mauríce Demarty, Camille Ripoll

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Abstract

SUMMARY In this paper, we report that Erwinia carotovora can perform controlled biological pectinolysis of green flax fibre. The degradation of pectic substances is considered a key step during this process and is estimated by determining the cation exchange capacity (CEC). A CEC characteristic of a ‘good retting degree’, is reached after 20 h incubation, whatever the tested temperature and initial pectate lyase activity of the bacterial culture. Similar results are obtained after only 4 h, at a temperature of 45°C and the pectate lyase activity is greater than 2 nmol ml‐1 min‐1. The linear relationship between the CEC and the weight loss of green fibre which is established, allows us to estimate an equivalent weight of 1000 g eq‐1 for the solubilised polymers.

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SUMMARY In this paper, we report that Erwinia carotovora can perform controlled biological pectinolysis of green flax fibre. The degradation of pectic substances is considered a key step during this process and is estimated by determining the cation exchange capacity (CEC). A CEC characteristic of a ‘good retting degree’, is reached after 20 h incubation, whatever the tested temperature and initial pectate lyase activity of the bacterial culture. Similar results are obtained after only 4 h, at a temperature of 45°C and the pectate lyase activity is greater than 2 nmol ml‐1 min‐1. The linear relationship between the CEC and the weight loss of green fibre which is established, allows us to estimate an equivalent weight of 1000 g eq‐1 for the solubilised polymers.

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Available abstract

SUMMARY In this paper, we report that Erwinia carotovora can perform controlled biological pectinolysis of green flax fibre. The degradation of pectic substances is considered a key step during this process and is estimated by determining the cation exchange capacity (CEC). A CEC characteristic of a ‘good retting degree’, is reached after 20 h incubation, whatever the tested temperature and initial pectate lyase activity of the bacterial culture. Similar results are obtained after only 4 h, at a temperature of 45°C and the pectate lyase activity is greater than 2 nmol ml‐1 min‐1. The linear relationship between the CEC and the weight loss of green fibre which is established, allows us to estimate an equivalent weight of 1000 g eq‐1 for the solubilised polymers.

Key concepts: Retting, Pectate lyase, Erwinia, Biology, Degradation (telecommunications), Incubation, Botany, Food science

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