M Rønne, Kirsten Vang Nielsen, Mogens Erlandsen
Abstract
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When human lymphoid cells are exposed to colcemid overnight at 4°C and subsequently incubated at 37.5°–38°C, variation in incubation time offers a simple method to produce chromosome slides with a high frequency of either prophase, prometaphase, or metaphase chromosomes.
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When human lymphoid cells are exposed to colcemid overnight at 4°C and subsequently incubated at 37.5°–38°C, variation in incubation time offers a simple method to produce chromosome slides with a high frequency of either prophase, prometaphase, or metaphase chromosomes.
Key concepts: Colcemid, Prometaphase, Metaphase, Biology, Prophase, Chromosome, Molecular biology, Genetics