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Ribosome Purification by Isopycnic Sedimentation in High Density Sucrose Gradients

Paul Nieuwenhuysen, Julius Clauwaert

Abstract

The applicability has been investigated of a method for the isolation of cytoplasm (and mitochondrial) ribosomes of various eukaryotes. The procedure is based on centrifugation techniques and includes a sedimentation of the ribosomes in a sucrose gradient of very high density. It was found to be particularly useful with material which contains relatively few ribosomes.

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Available abstract

Key concepts: Ribosome, Isopycnic, Sucrose gradient, Density gradient, Sedimentation, Cytoplasm, Differential centrifugation, Centrifugation