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Determination of Chondroitin‐4‐Sulfate through Its Uronic Acid Content

B. Zsadon, K. Horváth‐Otta, Ferenc Tüdős, É. Dósa, J�zsef Szejtli

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Abstract

Abstract A standardized new variant of the uronic acid determination based on carbazole reaction is suggested which has been proved for routine analyses. Its relative error is less than ± 2% in case of measuring 50–340 μg chondroitin‐4‐sulfate or the equivalent glucuronic acid. The method is suitable for determining other mucopolysaccharides, polyuronides and uronic acids, too.

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What this paper is about

Abstract A standardized new variant of the uronic acid determination based on carbazole reaction is suggested which has been proved for routine analyses. Its relative error is less than ± 2% in case of measuring 50–340 μg chondroitin‐4‐sulfate or the equivalent glucuronic acid. The method is suitable for determining other mucopolysaccharides, polyuronides and uronic acids, too.

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Available abstract

Abstract A standardized new variant of the uronic acid determination based on carbazole reaction is suggested which has been proved for routine analyses. Its relative error is less than ± 2% in case of measuring 50–340 μg chondroitin‐4‐sulfate or the equivalent glucuronic acid. The method is suitable for determining other mucopolysaccharides, polyuronides and uronic acids, too.

Key concepts: Uronic acid, Glucuronic acid, Chemistry, Glycosaminoglycan, Sulfate, Chondroitin sulfate, Chondroitin, Chromatography

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