1997•Journal of HistotechnologyRequires access

Modified Hematoxylin and Eosin Stain for Elastic Fibers in Rat Carotid and Pig Coronary Arteries After Balloon Injury

Maria T. Valentine, Tonya Jenkins-West, Mark C. Kowala, Patricia Ferrer

Abstract

We modified and automated a conventional hematoxylin and eosin stain for formalin fixed, paraffin embedded rat carotid and pig coronary arteries. This method enhanced the staining of the elastic laminae in injured pig coronary and rat carotid arteries, and improved morphometric measurements of the media and neointima by computerized image analysis. This modified hematoxylin and eosin stain can replace complicated elastic stains such as Verhoeff-Van Gieson or Movat pentachrome as a method for routine staining of arteries for morphometric analysis. (The J Histotechnol 20:61, 1997)

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Key concepts: H&E stain, Van Gieson's stain, Stain, Staining, Neointima, Pathology, Medicine, Carotid arteries