Optimization of the Method for Simultaneous Extraction Bioleaching Bacteria DNA and RNA

Metagenomic and metatrascription technologies were developed rapidly and applied widely in environmental microbiology in recent years and were also gradually applied in biohydrometallurgy. However, how to get good quality DNA and RNA simultaneously is still the key factor to determine the success and relibility of the whole data analysis. In this study, a wide used DNA extraction method [ was optimized for extraction of crude nucleic acid from acidic habitat. Our results showed that nucleic acid extraction buffer (pH 7.0) contain PIPES salt could effectively extract crude nucleic acid (including DNA and RNA) from four pure culture and several acidic environmental samples. Qiagen DNA and RNA isolation system (All prep DNA/RNA Mini kit) could separate DNA and RNA. Good quality with A260/280 was around 1.87±0.01 for total 33.88±7.85 μg DNA and A260/230 around 2.07±0.07 for total 20.00±2.73 μg RNA were obtained from 300 mL A.f culture medium (strain concentration was around 2×107). All isolated DNA and RNA could be used for downstream molecular analysis. This study provides a useful method for simultaneous extraction, separation and purification DNA and RNA from acidic habitat, which could accelerate the quantitative analysis for biohydrometually and therefore increase the efficiency of biohydrometually.