2014•Chemical CommunicationsOpen access

Pyrazine-derived disulfide-reducing agent for chemical biology

John C. Lukesh, Kelly K. Wallin, Ronald T. Raines

Abstract

For fifty years, dithiothreitol (DTT) has been the preferred reagent for the reduction of disulfide bonds in proteins and other biomolecules. Herein we report on the synthesis and characterization of 2,3-bis(mercaptomethyl)pyrazine (BMMP), a readily accessible disulfide-reducing agent with reactivity under biological conditions that is markedly superior to DTT and other known reagents.

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Key concepts: Dithiothreitol, Reducing agent, Reagent, Pyrazine, Disulfide bond, Biomolecule, Chemistry, Combinatorial chemistry