1995•European Journal of BiochemistryRequires access

Analysis of the Iron‐Sulfur Clusters Within the Complex I (NADH: Ubiquinone Oxidoreductase) Isolated from Potato Tuber Mitochondria

Tse‐I Lin, Vladimir D. Sled, Tomo̧ko Ohnishi, Axel Brennicke, Lutz Grohmann

Abstract

The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.

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Key concepts: Neurospora crassa, Oxidoreductase, Iron–sulfur cluster, Sulfur, Chemistry, Solanum tuberosum, Sulfide, Electron paramagnetic resonance