2004•Journal of Pharmacological SciencesOpen access
Expression of CYP3A4 mRNA Is Correlated With CYP3A4 Protein Level and Metabolic Activity in Human Liver
Minoru Watanabe, Toshio Kumai, Naoki Matsumoto, Masami Tanaka, Satoshi O. Suzuki, Tetsuo Satoh, Shinichi Kobayashi
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Abstract
A relatively large amount of human liver tissue was required to determine the exact activity of human hepatic CYP3A. Although, the quantity of available human liver tissue samples is limited. We measured levels of CYP3A4 mRNA by RT-PCR with a radiolabeled primer specific for CYP3A4 and compared mRNA expression with CYP3A4 protein level and metabolic activity in liver. The level of CYP3A4 mRNA was correlated with the levels of CYP3A4 protein and activity. Our results suggest that CYP3A4 protein and activity levels can be predicted from CYP3A4 mRNA levels determined by RT-PCR and using a very small amount of liver tissue.
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