Changes in Serum Proteins by Albumin-and Globulinpheresis

In order to obtain some additional findings in the research for the formation of plasma proteins, authors have carried out two types of experiments with dogs and rabbits which might be called albuminphersis and globulinpheresis. These have the similar character as plasmapheresis performed by Whipple and others. In alouminpheresis or globulinpheresis, a certain volume of blood was withdrawn from animals, and blood cells were separated from plasma by centrifugation, washed twice with saline, suspended in globulin or albumin solution respectively which had been previously prepared from pooled plasma of the same species, and the resulting mixture, having approximately the same volume and concentration of globulin or albumin as depleted blood, was injected back into amimals intravenously.By these procedures, animals were brought under the condition in which albumin or globulin fraction was partially lost while other blood components were remained almost unchanged. Changes in serum proteins after these treatments were analyzed at intervals by the micro-Kjeldahl method and the electrophoresis using a Tiselius apparatus.In this report, it has been shown that the decrease in albumin and the increase in α- and β-globulin have been observed with some differences in details between albumin- and globulinpheresis. Furthermore, on the basis of the results obtained, some discussions have been made about the formation of serum proteins in relation to the labile tissue protein.