2000•Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIERequires access
New spectrophotometer for easy absorbance measurement
Takeshi Taguchi, Mitsuo Hiramatsu
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Abstract
The spectrophotometer we have developed enables us to measure absorbance in a simple way without recovering and contaminating the analyte. The absorbance of the analyte can be measured with the sample solution being retained in the tip of the pipette. The traditional cuvette is totally unnecessary. Both the tip and pipette used in this spectrophotometer are commercially available. We have measured the absorbance of analytes containing DNA dissolved in water and compared them with results obtained by the conventional spectrophotometer. Both absorbance values sufficiently agree in repeated tests. The paper will present the features of our instrument and detailed results.