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Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease1

Makoto Komiyama, Tetsuro Shiiba, Yo-ta Takahashi, N. Takeda, Kazunari Matsumura, Teruyuki Kodama

Open publisher page 36 citations

Abstract

Cerium(IV) ion is attached to the 5′-end of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3′-5′)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

About this research paper

What this paper is about

Cerium(IV) ion is attached to the 5′-end of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3′-5′)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

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OpenAlex reports 36 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Cerium(IV) ion is attached to the 5′-end of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidylyl(3′-5′)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

Key concepts: Phosphodiester bond, Chemistry, Nuclease, Cerium, DNA, Hydrolysis, Thymidine, Stereochemistry

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