Long-term persistence of subterranean clover Trifolium subterraneum L.) cultivars at three sites in south-western Victoria

Summary. The seed banks of 6 subterranean clover ( Trifolium subterraneum L.) cultivars were sampled at 3 abandoned cultivar evaluation experiments in south-western Victoria (Hamilton, Macarthur and Timboon). Two were var. yanninicum (Trikkala and Larisa) and 4 were var. subterraneum (Denmark, Goulburn, Karridale and Leura). Seed was sampled in late summer–early autumn 1993, between 6 and 10 years after the sites were established and between 3 and 7 years after the sites were abandoned. The collected seed was separated into black and white seed, and a sample of the black seed was grown in a glasshouse and identified as either belonging to the sown cultivar or belonging to another cultivar/genotype. All white seed was assumed to belong to the sown cultivar. The aim was to determine if these widely used cultivars were persisting under farm management conditions and competition from perennial grass, other subterranean clover and annual weeds. With some variation between sites all cultivars were found to be persisting satisfactorily. Over the 3 sites, white-seeded cultivars averaged 460 kg/ha and 6640 seeds/m2; black-seeded cultivars averaged 260 kg/ha and 5590 seeds/m2. Contamination with other subterranean clover cultivars/ecotypes was generally low, except at Macarthur where the white-seeded cultivars were heavily contaminated (Trikkala 39% pure; Larisa 57% pure) with black seeds highlighting the poor adaptation of var. yanninicum to light soil types. Of the black seeds, over the 3 sites, average contamination level was 13% and ranged from 41% (Goulburn at Macarthur) to 1% (Leura at Timboon). The study indicates that in south-western Victoria, all the recently released cultivars are likely to persist and be productive, and that the small-plot evaluation techniques used to identify potential new cultivars are likely to be adequate if replicated in space and if the clovers are sown in mixed swards with perennial grass.