1990•Chinese Journal of ChemistryRequires access

Assignment of the non‐exchangeable proton resonances and conformational analysis of oligonucleotide DNA d(TTGGGGTT) by two‐dimensional nuclear magnetic resonance methods;

Yanhong Dong, Wen‐Xia Tang, Anbang Dai, Jia‐Zhen Wu, Jacques H. VAN BOOM, Jan Reedijk, A. F. Struik

Abstract

Abstract Two‐dimensional NMR study on the single‐stranded octadeoxynucleotide, d(TT‐GGGGTT) is presented. Assignments of the base protons, the H1′, H2′ and H2″ protons were made by two‐dimensional Nuclear Overhauser Enhancement Spectroscopy (NOESY) and two‐dimensional homonuclear J‐correlated Spectroscopy (COSY). The result indicates that the overall structure of the single‐stranded d (TTGGGGTT) is a right‐handed B‐type helix.

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Key concepts: Homonuclear molecule, Chemistry, Nuclear Overhauser effect, Two-dimensional nuclear magnetic resonance spectroscopy, Nuclear magnetic resonance spectroscopy, Nuclear magnetic resonance, Spectroscopy, Proton