The comparison study of establishing animal model of human uterine cancer using matrigel

The human endometrial adenocarcinoma cell line-Ishikawa cells suspended either in Matrigel or MEM buffer medium were injected subcutaneously to nude mice (N=10 mice/group); and the fresh human cervical cancer tissues obtained directly from the surgery were coated by Matrigel and transplanted into nude mouse. The purpose of these studies were to see if Matrigel could enhance the human uterine cancers’ proliferation and differentiation in vivo . The results showed that when cancer cells or tissues implanted into nude mice in Matrigel, the xenografts could turn out earlier, proliferate faster and kept the original differentiation better. It is suggested that Matrigel is an ideal agent in establishing animal models of human uterine cancer.