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2013•Advanced materials researchOpen access

Research on the Isolation, Identification and Degradation Characteristics of a Diesel Oil Degrading Strain

Lei Huang, Jing Chao Xie, Xiao Feng Shi, Jing Yan Lian

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Abstract

A hydrocarbon-degrading strain ZRS was isolated from petroleum-contaminated soils sampled from Xinjiang oil field and identified as Acinetobacter beijerinckii, which could use diesel oil as solo carbon source. The optimal temperature and pH for strain utilizing ethanol was 25°C and 7.2; the optimal concentration of ethanol and the biomass concentration was 4.5% and 109 CFU/mL, respectively. Inoculated to artificial seawater which added (NH4)2SO4 2.64g/L、Na2HPO4 1.5 g/L and yeast extract 13 mg/L after 7 days of culture at temperature 25°C, the rate of degradation was 73.9％.

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What this paper is about

A hydrocarbon-degrading strain ZRS was isolated from petroleum-contaminated soils sampled from Xinjiang oil field and identified as Acinetobacter beijerinckii, which could use diesel oil as solo carbon source. The optimal temperature and pH for strain utilizing ethanol was 25°C and 7.2; the optimal concentration of ethanol and the biomass concentration was 4.5% and 109 CFU/mL, respectively. Inoculated to artificial seawater which added (NH4)2SO4 2.64g/L、Na2HPO4 1.5 g/L and yeast extract 13 mg/L after 7 days of culture at temperature 25°C, the rate of degradation was 73.9％.

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Available abstract

A hydrocarbon-degrading strain ZRS was isolated from petroleum-contaminated soils sampled from Xinjiang oil field and identified as Acinetobacter beijerinckii, which could use diesel oil as solo carbon source. The optimal temperature and pH for strain utilizing ethanol was 25°C and 7.2; the optimal concentration of ethanol and the biomass concentration was 4.5% and 109 CFU/mL, respectively. Inoculated to artificial seawater which added (NH4)2SO4 2.64g/L、Na2HPO4 1.5 g/L and yeast extract 13 mg/L after 7 days of culture at temperature 25°C, the rate of degradation was 73.9％.

Key concepts: Diesel fuel, Strain (injury), Biomass (ecology), Ethanol, Degradation (telecommunications), Hydrocarbon, Chemistry, Yeast extract

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