Genotypic Variation in Geographical Isolates of Oryctes Baculovirus

Summary Twelve geographical isolates of Oryctes baculovirus were cloned in DSIR-HA-1179 cells. The DNA of these isolates was analysed by restriction endonuclease digestion with the enzymes BamHI, EcoRI, HindIII and PstI. Each isolate showed slightly different restriction fragment electrophoresis profiles. Most of the changes were due to small insertions or deletions of DNA, although two isolates lacked a single restriction site. The sites of genotypic change were not randomly distributed but were mainly associated with regions that have been shown to contain reiterated sequences.