Comparison of the chemical components of essential oil extracted by MAHD and SPME methods from two species of Salvia from Iran

Salvia macrosiphon Boiss. and Salvia rhytidea Benth. species of the genus Salvia which are belong to the Lamiaceae family. At the Present report deals with the analysis comparison of the essential oils from aerial parts of S. macrosiphon and S. rhytidea growing wild in Iran obtained by hydrodistillation (HD) , microware assisted hydrodistillation (MAHD) and solid phase micro extraction (SPME) procedures and the collected essential oils was investigated by GC and GC-MS. The major compounds of essential oil of S. macrosiphon extracted by hydrodistillation were: Linalool(12.6%),Caryophyllene oxide(11.9%) , Octane(8.5%) and β-Eudesmol(7.1%). Also the main compounds of essential oil from S. macrosiphon extracted by microwave assisted hydrodistillation were: Caryophyllene oxide(21.8%) ,Linalool(18.8%) and β-Eudesmol (6.1%). In this study,the gas chromatogram was obtained of injection the essential oil from aerial parts of S.macrosiphon extracted by solid phase micro extraction ,was not helpful. Chemical composition of volatile compounds from dried aerial parts of Salvia rhytidea Benth. was extracted, by hydrodistillation and solid phase micro extraction methods but microwave assisted hydrodistillation method was not helpful . The major compounds of essential oil of S.rhytidea extracted by hydrodistillation were:Sabinene(12.8%),Spathulenol(12.4%),Germacren-D (6.7%) , β-Eudesmol (6.3%) and α - Cubebene(5.4%). Also the main compounds of essential oil from S. rhytidea extracted by solid phase micro extraction were: Sabinene (38.5%), Limonene(11.6%) , Myrcene (7.2%) and β-Pinene(5.5%).DOI: http://dx.doi.org/10.3126/ijls.v9i2.12051 International Journal of Life Sciences 9 (2) : 2015; 61-64