Increased Digestibility of Raw Starches by Mutant Strains of Aspergillus awamori.
Aryanti Amsal, Machiko Takigami, Hitoshi Ito
Abstract
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Aryanti Amsal, Machiko Takigami, Hitoshi Ito
Abstract
Open-access reader
Aspergillus awamori possesses the ability to digest raw starches. For the effective utilization of starchy crops in South-Asian countries, it is important to digest raw starches without cooking for the industrial fermentation process. In this study, mutant strains of Aspergillus awamori IFO4033 with increased activity of α-amylase were isolated from freeze dried spores irradiated with C5+ ion-beams. The frequency of the mutation and the activity were higher for C5+-irradiated samples than those for gamma-irradiated ones. Compared to the digestibility of raw starches from cassava, sago and sukun by the parent strain, it increased two- to threefold by some mutant strains obtained from C5+ ion-beam irradiated spores.
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Aspergillus awamori possesses the ability to digest raw starches. For the effective utilization of starchy crops in South-Asian countries, it is important to digest raw starches without cooking for the industrial fermentation process. In this study, mutant strains of Aspergillus awamori IFO4033 with increased activity of α-amylase were isolated from freeze dried spores irradiated with C5+ ion-beams. The frequency of the mutation and the activity were higher for C5+-irradiated samples than those for gamma-irradiated ones. Compared to the digestibility of raw starches from cassava, sago and sukun by the parent strain, it increased two- to threefold by some mutant strains obtained from C5+ ion-beam irradiated spores.
Key concepts: Aspergillus awamori, Food science, Spore, Mutant, Fermentation, Chemistry, Raw material, Aspergillus