2006•Letters in Applied MicrobiologyRequires access

Evaluation of a new chromogenic agar medium for isolation and identification of Group B streptococci

John David Perry, Michelle Oliver, Audrey Nicholson, Josephine A. Wright, Frances K. Gould

Abstract

AIMS: To evaluate a new chromogenic agar as a screening medium for the isolation of Group B streptococci from high vaginal swabs from pregnant women. METHODS AND RESULTS: The medium was evaluated with 195 high vaginal swabs referred for antenatal screening and compared with blood agar and Granada medium. The new chromogenic medium showed 100% sensitivity for the detection of Group B streptococci, and also showed a positive predictive value of 100%. Granada medium also showed excellent sensitivity and specificity and both media were superior to blood agar. CONCLUSIONS: The new chromogenic medium showed excellent performance for the detection of Group B streptococci from clinical samples. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first chromogenic medium described for the detection of Group B streptococci. The medium offers an effective and convenient alternative to conventional media, currently used in clinical laboratories.

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Key concepts: Chromogenic, Agar, Group B, Agar plate, Microbiology, Streptococcus agalactiae, Isolation (microbiology), Group A