A Time and Cost-Effective Assay for the Determination of Residues of Malachite Green in Fish Tissues by HPLC

A time and cost-effective method for the extraction and determination of residues of malachite green in fish tissue, muscle and liver, by HPLC is presented. The calibration curves were linear, and the recovery of malachite green was 101-116 %. The detection limits were 1 and 10 ng/g for malachite green in muscle and liver, respectively. A longer time of sample exposure to the extraction fluids was required to extract malachite green from samples from treated fish than from samples to which a standard amount of malachite green had been added. We suggest that samples be exposed to the extraction solvents overnight for extraction of residues of malachite green from fish tissue.