A Field Inoculation Method for Septoria tritici and Septoria nodorum
B. M. Cooke, Donovan Jones
Abstract
B. M. Cooke, Donovan Jones
Abstract
SUMMARY A method is described, using a Czapek Dox V‐8 agar and continuous irradiation with near‐ultraviolet light, which enables large numbers of spores of Septoria tritici and Septoria nodorum to be produced rapidly and also allows easy identification of the two pathogens by their colony morphology. Also, a simple field inoculation method is described which has proved successful both in experiments involving different inoculum concentrations and for the screening of spring and winter wheat cultivars for their reactions against both pathogens.
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SUMMARY A method is described, using a Czapek Dox V‐8 agar and continuous irradiation with near‐ultraviolet light, which enables large numbers of spores of Septoria tritici and Septoria nodorum to be produced rapidly and also allows easy identification of the two pathogens by their colony morphology. Also, a simple field inoculation method is described which has proved successful both in experiments involving different inoculum concentrations and for the screening of spring and winter wheat cultivars for their reactions against both pathogens.
Key concepts: Septoria, Biology, Spore, Inoculation, Cultivar, Agar, Botany, Horticulture