Antioxidant and Antimicrobial Activities of Callus Culture and Fruits ofPhyllanthus emblica L.
Bharat B. Singh, Ram Avtar Sharma
Abstract
Bharat B. Singh, Ram Avtar Sharma
Abstract
The antioxidant activity of the callus of Phyllanthus emblica was tested by using DPPH− radical scavenging, reducing power, superoxide anion, and metal chelating scavenging models. Digallic acid was the major compound in quantity both in vivo (2.61 ± 0.887%) and in vitro (2.89 ± 0.997%) in 6-week-old cell cultures. Digallic acid demonstrated maximum antioxidant activity against the antioxidant systems, while other compounds exhibited mild-to-moderate antioxidant activity. Among the isolated phytochemicals, putranjivain-A showed maximum activity against Escherichia coli (85 μg.mL−1) and Rhizoctonia phaseoli (70 μg.mL−1).
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The antioxidant activity of the callus of Phyllanthus emblica was tested by using DPPH− radical scavenging, reducing power, superoxide anion, and metal chelating scavenging models. Digallic acid was the major compound in quantity both in vivo (2.61 ± 0.887%) and in vitro (2.89 ± 0.997%) in 6-week-old cell cultures. Digallic acid demonstrated maximum antioxidant activity against the antioxidant systems, while other compounds exhibited mild-to-moderate antioxidant activity. Among the isolated phytochemicals, putranjivain-A showed maximum activity against Escherichia coli (85 μg.mL−1) and Rhizoctonia phaseoli (70 μg.mL−1).
Key concepts: Phyllanthus emblica, Antioxidant, DPPH, Antimicrobial, Callus, Chemistry, Phyllanthus, Traditional medicine