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2006•Cold Spring Harbor ProtocolsRequires access

Staining SDS-Polyacrylamide Gels with Silver Salts

Joseph Sambrook, David W. Russell

Open publisher page 4 citations

Abstract

INTRODUCTIONA number of methods have been developed to stain polypeptides with silver salts after separation by SDS-polyacrylamide gel electrophoresis. It is somewhat more difficult to perform than staining with Coomassie Brilliant Blue R-250, but it is ~100-1000-fold more sensitive and is capable of detecting as little as 0.1-1.0 ng of polypeptide in a single band.

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INTRODUCTIONA number of methods have been developed to stain polypeptides with silver salts after separation by SDS-polyacrylamide gel electrophoresis. It is somewhat more difficult to perform than staining with Coomassie Brilliant Blue R-250, but it is ~100-1000-fold more sensitive and is capable of detecting as little as 0.1-1.0 ng of polypeptide in a single band.

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Available abstract

INTRODUCTIONA number of methods have been developed to stain polypeptides with silver salts after separation by SDS-polyacrylamide gel electrophoresis. It is somewhat more difficult to perform than staining with Coomassie Brilliant Blue R-250, but it is ~100-1000-fold more sensitive and is capable of detecting as little as 0.1-1.0 ng of polypeptide in a single band.

Key concepts: Silver stain, Staining, Coomassie Brilliant Blue, Polyacrylamide, Polyacrylamide gel electrophoresis, Stain, Chromatography, Chemistry

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