1987•ScienceRequires access
Human CSF-1: Molecular Cloning and Expression of 4-kb cDNA Encoding the Human Urinary Protein
Gordon Wong, Patricia A. Temple, Anne C. Leary, J Witek-Giannotti, Yu‐Chung Yang, A. Ciarletta, Margaret P. Chung, Patricia E. Murtha, Ronald Kriz, Randal J. Kaufman, Catherine R. Ferenz, Barbara S. Sibley, Katherine J. Turner, Rodney M. Hewick, Steven C. Clark, Nobuya Yanai, Hajime Yokota, Muneo Yamada, Minoru Saito, Kazuo Motoyoshi, Fumimaro Takaku
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Abstract
A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.