Naloxone affects reproductive system in a rat model with polycystic features

To make interaction between morphine and naloxone in the rat model of PCOS, we evidenced the opioid receptors involvement in this efficacy. A total of 48 female animals (Wistar rats weighing 200–250 g) were kept diestrous before experimental procedure began. They were grouped in single L-arginine (50 mg/kg, i.p., once a day for 9 days), naloxone (0.4 mg/kg, i.p., once daily for 9 days), morphine (5 mg/kg, i.p., once a day for 9 days), and naloxone (0.4 mg/kg) pre-treated to L-arginine (50 mg/kg), morphine (5 mg/kg) pre-treated to L-arginine, morphine pre-treated to the collective naloxone-L-arginine. Control group solely received saline (1 mL/kg, once daily for 9 days). At the end of the treatment period all animals were surgically studied. The rats' ovaries and uteri were examined both biometrically and pathologically. The ovaries of rats treated with L-arginine showed polycystic characteristics and their uteri illustrated inflammation changes to the controls. The samples obtained from rats pre-treated with naloxone revealed a decrease in sign of inflammation compaired with L-arginine received speciments, the signs got worse in the presence of the morphine. Aspect of rat reproductive system may be linked with the cystic characteristic of ovary. This study involves opioid receptors in the naloxone efficacy on reproductive agents of rat with polycystic aspect.