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1967•European Journal of BiochemistryRequires access

The Standard Redox Potential of Cysteine-Cystine from the Thiol-Disulphide Exchange Reaction with Glutathione and Lipoic Acid

P. C. Jocelyn

Open publisher page 271 citations

Abstract

Thiol-disulphide exchange between glutathione (GSH) and cystine (CSSC) or oxidised glutathione (GSSG) and cysteine (CSH) has been followed by assaying GSH and GSSG specifically at low pH during the reaction. Velocity and equilibrium constants have been derived. From the latter and the known standard oxidation-reduction potential (E0′) of GSH/GSSG, E0′ (pH 7) of CSH/CSSC is found to be -0.22 volt. The course of the reaction of both these pairs with dihydrolipoic acid/lipoic acid has been inferred from absorbance changes at 330 mμ. Results confirm that their E0′ values are considerably more positive than for the dihydrolipoic acid/lipoic acid system.

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What this paper is about

Thiol-disulphide exchange between glutathione (GSH) and cystine (CSSC) or oxidised glutathione (GSSG) and cysteine (CSH) has been followed by assaying GSH and GSSG specifically at low pH during the reaction. Velocity and equilibrium constants have been derived. From the latter and the known standard oxidation-reduction potential (E0′) of GSH/GSSG, E0′ (pH 7) of CSH/CSSC is found to be -0.22 volt. The course of the reaction of both these pairs with dihydrolipoic acid/lipoic acid has been inferred from absorbance changes at 330 mμ. Results confirm that their E0′ values are considerably more positive than for the dihydrolipoic acid/lipoic acid system.

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Available abstract

Thiol-disulphide exchange between glutathione (GSH) and cystine (CSSC) or oxidised glutathione (GSSG) and cysteine (CSH) has been followed by assaying GSH and GSSG specifically at low pH during the reaction. Velocity and equilibrium constants have been derived. From the latter and the known standard oxidation-reduction potential (E0′) of GSH/GSSG, E0′ (pH 7) of CSH/CSSC is found to be -0.22 volt. The course of the reaction of both these pairs with dihydrolipoic acid/lipoic acid has been inferred from absorbance changes at 330 mμ. Results confirm that their E0′ values are considerably more positive than for the dihydrolipoic acid/lipoic acid system.

Key concepts: Glutathione, Chemistry, Cystine, Cysteine, Lipoic acid, Thiol, Redox, Cysteamine

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The Standard Redox Potential of Cysteine-Cystine from the Thiol-Disulphide Exchange Reaction with Glutathione and Lipoic Acid — Research Paper | ScholarLens