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Ribosome Biogenesis: Nonrandom Addition of Structural Proteins to 50 S Subunits

B.H. Sells, Francis C. Davis

Abstract

Assemblage of structural proteins into 50S subunits was examined in Escherichia coli recovering fromt chloramphenicol treatment. Cells previously labeled with H3-leucine for three generations were incuibated for 30 minutes with chloramnlphenicol. Proteins synthesized during the initial 5 minuites of recovery from chlorarmphenicol treatmnent were labeled with C(14)-leucine. Marked variation in the ratios of C(14)- to H(3)-leucine in ribosomal protein occurred in cells that had been treated with chloramphenicol; luntreated cells displayed little variation. Thle resuilts sliggest that ribosomal proteins are assenmbled into 50S subunits in a nonrandom manner.

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Key concepts: Chloramphenicol, 50S, Ribosome, Ribosomal protein, Leucine, Ribosomal RNA, Escherichia coli, Protein subunit