2003•Plant BreedingRequires access

Identification of a molecular marker linked to the closed capsule mutant trait in sesame using AFLP

Bülent Uzun, D. Lee, P. Donini, M. L ÇAǧirgan

Abstract

Abstract The identification of an amplified fragment length polymorphism (AFLP) marker linked to an agronomically useful trait in sesame is reported. A bulked segregant analysis (BSA) approach was adopted on segregating progenies of a cross between the closed capsule mutant line ‘cc3’, and the Turkish variety ‘Muganli‐57′. A total of 72 primer combinations were screened for linkage to the trait, but only one closely linked amplified fragment length polymorphism (AFLP) marker was identified. The linkage was confirmed by analysing the AFLP profile from single plants. The marker has the potential to accelerate breeding programmes aimed at modifying unwanted side‐effects of the closed capsule mutation by marker‐assisted selection.

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Key concepts: Amplified fragment length polymorphism, Bulked segregant analysis, Biology, Genetics, Genetic marker, Marker-assisted selection, Molecular marker, Primer (cosmetics)