2007•Biotechnology and Applied BiochemistryRequires access
Expression of human acidic fibroblast growth factor in Nicotiana benthamiana with a potato‐virus‐X‐based binary vector
Jingying Liu, Pengda Ma, Yan Sun, Meiying Yang, Liping Yang, Yuxi Li, Yanfang Wu, Xiaojuan Zhu, Xingzhi Wang
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Abstract
The aFGF (acidic fibroblast growth factor) plays an important role in morphogenesis, angiogenesis and wound healing and is therefore of potential medical interest. A DNA fragment encoding haFGF (human aFGF) has been cloned into the PVX (potato virus X)-based binary vector (pgR107) and transiently expressed in Nicotiana benthamiana (a wild Australian tobacco) by agroinfection. Approx. 1 week after agroinfection, the recombinant haFGF accumulated in the agroinfected plants reached up to 1% of the total soluble protein. haFGF was then purified on heparin-Sepharose CL-6B. The purified haFGF could stimulate the growth of NIH 3T3 cells, suggesting that the recombinant haFGF expressed via PVX viral vector in N. benthamiana was active biologically.