1975•Agricultural and Biological ChemistryOpen access

Purification, crystallization, and properties of an endo .BETA.-1,3-glucanase from Rhizopus chinensis R-69.

Shinpei Yamamoto, Susumu Nagasaki

Abstract

An endo ƒÀ-1,3-glucanase was purified in crystalline form from a culture filtrate of Rhizopus chinensis R-69.Molecular weight of the enzyme was determined to be 23,000 by molecular sieve chromatography and the mode of action of the enzyme was suggested to be a less random type of ƒÀ-1,3-glucanase.Km and Vmaa of the enzyme for laminarin are 3.4g/liter and 154 L U.,

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Available abstract

Key concepts: Laminarin, Glucanase, Rhizopus, Chemistry, Enzyme, Yeast, Rhizopus arrhizus, Molecular sieve