1989•Journal of Pharmacobio-DynamicsOpen access

Induction of glutathione S-transferase by phenobarbital in rat hepatocyte culture.

Haruyo Sasaki, Shuichi Miyaura, Kiyoko HORIE, Hideo Isono

Abstract

The in vitro effect of phenobarbital on glutathione S-transferase (GST) activity toward 1-chloro-2,4-dinitrobenzene (CDNB) in rat hepatocyte culture was investigated. Treatment of hepatocytes with a medium containing 6 or 8 mM phenobarbital for 20 h increased the specific activity of GST approx. 140% and 160% of control, respectively. When induction of GST by phenobarbital treatment was observed, the cellular level of phenobarbital determined by HPLC was approx. 10 - 15 micrograms/mg of 20000 x g supernatant protein of hepatocytes. Thus, an inductive effect of phenobarbital on GST was observed in vitro as well as in rat liver. It was suggested that a certain level of cellular phenobarbital was necessary for the induction.

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Key concepts: Phenobarbital, Hepatocyte, Glutathione, In vitro, Enzyme inducer, Chemistry, Glutathione S-transferase, Biochemistry