1977•Proceedings of the Japan Academy Series BOpen access
A Simple Giemsa Method for the Differential Staining of Sister Chromatids with a Note on the Presumptive Mechanism Involved
Shunichi Sakanishi, Susumu Takayama
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Abstract
Chinese hamster strain cells were grown in the presence of BrdU (10μM final concentration) for 26hr. The cells were ordinarily air-dried, and the slides were incubated in Na2HPO4 (1.0M) at 75°C for 2 minutes. After Giemsa staining, thee treated chromosomes showed a clear differential staining between their sister chromatids. Results of the examination by the use of fluorescent Feulgen reaction suggested that the chromatid which stained weakly with Giemsa contains less DNA than the chromatid which stained darkly.
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