1987Agricultural and Biological ChemistryOpen access

Glycerol metabolism in methylotrophic yeasts.

Yoshiki Tani, Keiko Yamada

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Abstract

Enzymeactivities involved in the initial step of glycerol metabolism were determined in cells of methylotrophic yeasts grown on glycerol, methanol or glucose.In Candida boidinii (Kloeckera sp.) No. 2201 , the activities of glycerol kinase and dihydroxyacetone kinase were detected in cells grown on glycerol and methanol, respectively.The activity of NAD+-linked glycerol dehydrogenase of Hansenula polymorpha DL-1 was induced by glycerol and methanol, while that of Hansenula ofunaensis was induced by glycerol.The enzymes of both strains were subject to catabolite

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Enzymeactivities involved in the initial step of glycerol metabolism were determined in cells of methylotrophic yeasts grown on glycerol, methanol or glucose.In Candida boidinii (Kloeckera sp.) No. 2201 , the activities of glycerol kinase and dihydroxyacetone kinase were detected in cells grown on glycerol and methanol, respectively.The activity of NAD+-linked glycerol dehydrogenase of Hansenula polymorpha DL-1 was induced by glycerol and methanol, while that of Hansenula ofunaensis was induced by glycerol.The enzymes of both strains were subject to catabolite

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Available abstract

Enzymeactivities involved in the initial step of glycerol metabolism were determined in cells of methylotrophic yeasts grown on glycerol, methanol or glucose.In Candida boidinii (Kloeckera sp.) No. 2201 , the activities of glycerol kinase and dihydroxyacetone kinase were detected in cells grown on glycerol and methanol, respectively.The activity of NAD+-linked glycerol dehydrogenase of Hansenula polymorpha DL-1 was induced by glycerol and methanol, while that of Hansenula ofunaensis was induced by glycerol.The enzymes of both strains were subject to catabolite

Key concepts: Glycerol, Glycerol kinase, Catabolite repression, Dihydroxyacetone, Biochemistry, Chemistry, Oxidative phosphorylation, Enzyme

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