2002•South African Journal of Plant and SoilOpen access

AFLP and RAPD markers linked to leaf rust resistance geneLr41in wheat

J-M. Lottering, Anna‐Maria Botha, F. J. Kloppers

Abstract

RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) analyses were used to identify molecular markers linked to the leaf rust resistance gene Lr41. Bulk and parental lines were screened with 380 RAPD primers and 64 AFLP primer combinations. A total of 3190 RAPD and 1859 AFLP loci were screened for linkage to the resistance gene. Twelve fragments, seven RAPD and five AFLP, were identified as putative markers for the leaf rust resistance gene Lr41. Nine of these were linked in coupling phase and three in repulsion phase. Segregation analysis of these markers in a BC2F2 population, segregating for the resistance gene, revealed varying linkage distances that ranged from 2.8 to 29 cM. The coupling phase AFLP marker E-AAG:M-CTA300c was converted into a sequence characterized amplified region (SCAR) marker.

Open-access reader

About this research paper

What this paper is about

Why it matters

OpenAlex reports 5 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Amplified fragment length polymorphism, RAPD, Biology, Genetics, Genetic marker, Molecular marker, Gene, Genetic linkage