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1986•Canadian Journal of MicrobiologyRequires access

Purification of the thermostable direct hemolysin of Vibrio parahaemolyticus by immunoaffinity column chromatography

Takeshi Honda, Myonsun Yoh, Ikuyo Narita, T Miwatani, H. Sima, You Xiegen, Li Xiandi

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Abstract

A simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of Vibrio parahaemolyticus was developed. The thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of V. parahaemolyticus infection in China in 1985 was indistinguishable from the hemolysins purified from strains isolated in Japan.

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What this paper is about

A simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of Vibrio parahaemolyticus was developed. The thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of V. parahaemolyticus infection in China in 1985 was indistinguishable from the hemolysins purified from strains isolated in Japan.

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Available abstract

A simple method involving immunoaffinity column chromatography to purify the thermostable direct hemolysin of Vibrio parahaemolyticus was developed. The thermostable direct hemolysin purified from the culture supernatant of a strain isolated from the first reported case of V. parahaemolyticus infection in China in 1985 was indistinguishable from the hemolysins purified from strains isolated in Japan.

Key concepts: Vibrio parahaemolyticus, Hemolysin, Vibrionaceae, Affinity chromatography, Microbiology, Chromatography, Biology, Vibrio

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Purification of the thermostable direct hemolysin of Vibrio parahaemolyticus by immunoaffinity column chromatography — Research Paper | ScholarLens