Identification of a Highly Efficient Aerobic Denitrifying Bacterium in SBR and Denitrification Optimization

Aerobic denitrification process is attracted more attention to enhance nitrogen removal technology. Aerobic denitrifying bacteria present excelled abilities with fast growth rate and denitrifying speed, though they are not dominant in most practical processes. A high-efficiency strain was enriched in SBR from aerobic activated sludge by the way of intermittent aeration and continuous aeration combination. The strain was determined to Pseudomonasstutzeri T13 with ability of TN removal 90% and nitrate removal 97%. The limiting factors for aerobic denitrifying efficiency were optimized to temperature=31°C,pH=7.11 and DO=2.5 mg/L using response surface methodology. A total of 32 genes are related to nitrogen removal in strain T13. And 10 related to nitrate reductase, especially including 3 genes encoding the periplasmic nitrate reductase, playing important role to aerobic denitrification. It gave good understanding to supply effective technological supports for aerobic denitrification process.