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2005•Molecular Ecology NotesRequires access

Isolation and characterization of microsatellite DNA markers from mangrove crab, Scylla paramamosain

Masatsugu Takano, Anna Alexandrovna Barinova, Takuma Sugaya, Yasuhiro Obata, Tomohisa Watanabe, Minoru Ikeda, Nobuhiko Taniguchi

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Abstract

Abstract The first five variable microsatellite DNA loci for mangrove crab, Scylla paramanosain, were developed. Allelic variation and other characteristics at these loci were examined in this species captured at the Urado Bay, Japan. The number of alleles per locus ranged from 24 to 44. The expected heterozygosity across loci ranged from 0.900 to 0.999 and probability of identity (PI) ranged from 2.8 × 10−3 to 1.7 × 10−2. Therefore, these microsatellite markers could be useful for estimating effect of stock enhancement release and population genetic structure of mangrove crab.

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Abstract The first five variable microsatellite DNA loci for mangrove crab, Scylla paramanosain, were developed. Allelic variation and other characteristics at these loci were examined in this species captured at the Urado Bay, Japan. The number of alleles per locus ranged from 24 to 44. The expected heterozygosity across loci ranged from 0.900 to 0.999 and probability of identity (PI) ranged from 2.8 × 10−3 to 1.7 × 10−2. Therefore, these microsatellite markers could be useful for estimating effect of stock enhancement release and population genetic structure of mangrove crab.

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Available abstract

Abstract The first five variable microsatellite DNA loci for mangrove crab, Scylla paramanosain, were developed. Allelic variation and other characteristics at these loci were examined in this species captured at the Urado Bay, Japan. The number of alleles per locus ranged from 24 to 44. The expected heterozygosity across loci ranged from 0.900 to 0.999 and probability of identity (PI) ranged from 2.8 × 10−3 to 1.7 × 10−2. Therefore, these microsatellite markers could be useful for estimating effect of stock enhancement release and population genetic structure of mangrove crab.

Key concepts: Scylla paramamosain, Microsatellite, Biology, Loss of heterozygosity, Mangrove, Locus (genetics), Genetics, Allele

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