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A novel multistep method for generating precise unidirectional deletions using BspMI, a class-IIS restriction enzyme

Noaman M. Hasan, Sun C. Kim, Anna J. Podhajska, Waclaw Szybalski

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Key concepts: Biology, Restriction enzyme, pUC19, Multiple cloning site, Restriction site, Plasmid, Restriction fragment, Sticky and blunt ends

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A novel multistep method for generating precise unidirectional deletions using BspMI, a class-IIS restriction enzyme — Research Paper | ScholarLens